Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 77
... coli cells . The cells are spread onto an agar plate ; this physically separates the DNA fragments because different fragments are carried by different cells . The cells grow into colonies , and then a piece of filter paper is placed on ...
... coli cells . The cells are spread onto an agar plate ; this physically separates the DNA fragments because different fragments are carried by different cells . The cells grow into colonies , and then a piece of filter paper is placed on ...
Page 131
... coli cells , and some of the DNA molecules enter the bacteria . The task is to isolate a pure culture of bacterial cells harboring a plasmid that con- tains only a particular piece of rabbit cDNA ( hemoglobin cDNA ) . After the bacteria ...
... coli cells , and some of the DNA molecules enter the bacteria . The task is to isolate a pure culture of bacterial cells harboring a plasmid that con- tains only a particular piece of rabbit cDNA ( hemoglobin cDNA ) . After the bacteria ...
Page 132
... cells containing plasmids joined to rabbit DNA from those that do NOT have rabbit DNA attached to them ( Figure 8-2 ) ... coli cells are transformed with the recombinant DNA . ( d ) Plasmid - containing cells are selected by growth on agar ...
... cells containing plasmids joined to rabbit DNA from those that do NOT have rabbit DNA attached to them ( Figure 8-2 ) ... coli cells are transformed with the recombinant DNA . ( d ) Plasmid - containing cells are selected by growth on agar ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses