Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 46
A Guide for the Curious Karl Drlica. ( a ) Amino acid Enzyme Enzyme - amino acid complex ( b ) + ( c ) tRNA Enzyme - amino acid complex Enzyme - amino acid - tRNA complex Enzyme - amino acid - tRNA complex Enzyme Aminoacyl - tRNA Figure ...
A Guide for the Curious Karl Drlica. ( a ) Amino acid Enzyme Enzyme - amino acid complex ( b ) + ( c ) tRNA Enzyme - amino acid complex Enzyme - amino acid - tRNA complex Enzyme - amino acid - tRNA complex Enzyme Aminoacyl - tRNA Figure ...
Page 71
A Guide for the Curious Karl Drlica. HANDLING ENZYMES Thus far enzymes have been considered to be tiny agents that some ... enzyme of interest is separated from other cell components by a series of physical manipulations . Separa- tion is ...
A Guide for the Curious Karl Drlica. HANDLING ENZYMES Thus far enzymes have been considered to be tiny agents that some ... enzyme of interest is separated from other cell components by a series of physical manipulations . Separa- tion is ...
Page 105
... enzymes produces DNA fragments having discrete lengths . Enzymes can be obtained that recognize different sites in a DNA molecule , making it possible to cut DNA at a wide variety of locations . Cuts by different enzyme types produce ...
... enzymes produces DNA fragments having discrete lengths . Enzymes can be obtained that recognize different sites in a DNA molecule , making it possible to cut DNA at a wide variety of locations . Cuts by different enzyme types produce ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses