Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 99
... lambda ( Figure 6-4b ) . When lambda DNA is injected into a bacterial cell , it has two choices . It can behave as described above and destroy the bacterium . Alterna- tively , it can take up residence in the cell and enter a quiescent ...
... lambda ( Figure 6-4b ) . When lambda DNA is injected into a bacterial cell , it has two choices . It can behave as described above and destroy the bacterium . Alterna- tively , it can take up residence in the cell and enter a quiescent ...
Page 100
... lambda DNA replicates along with it . In this dor- mant state lambda DNA does little more than produce repressor pro- tein to keep its genes shut down . At the same time , the repressor protects the bacterial cell from infection by other ...
... lambda DNA replicates along with it . In this dor- mant state lambda DNA does little more than produce repressor pro- tein to keep its genes shut down . At the same time , the repressor protects the bacterial cell from infection by other ...
Page 128
... LAMBDA DNA Hemoglobin genes were initially separated from all the other genes in the DNA preparation by cloning them into bacteriophage lambda DNA ( see Chapter 6 for description of bacteriophages ) . The phage is particularly ...
... LAMBDA DNA Hemoglobin genes were initially separated from all the other genes in the DNA preparation by cloning them into bacteriophage lambda DNA ( see Chapter 6 for description of bacteriophages ) . The phage is particularly ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses