Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 139
... nucleotides long ) is synthesized which has the nucleotide sequence predicted from the amino acid sequence of the protein . This oligonucleo- tide is then radioactively labeled and used as a probe for detect- ing bacterial colonies or ...
... nucleotides long ) is synthesized which has the nucleotide sequence predicted from the amino acid sequence of the protein . This oligonucleo- tide is then radioactively labeled and used as a probe for detect- ing bacterial colonies or ...
Page 141
... DNA Overview Gene cloning technologies make it possible to obtain the large amounts of spe- cific regions of DNA necessary to study gene structure and function . Gene structure is analyzed by determining the nucleotide sequence of a section ...
... DNA Overview Gene cloning technologies make it possible to obtain the large amounts of spe- cific regions of DNA necessary to study gene structure and function . Gene structure is analyzed by determining the nucleotide sequence of a section ...
Page 150
... sequences . For ex- ample , the entire sequence for bacteriophage lambda DNA ( 48,498 nucleotides ) was determined in this way . Particular genes are located in the sequence by comparing the nucleotide sequence to that ex- pected from ...
... sequences . For ex- ample , the entire sequence for bacteriophage lambda DNA ( 48,498 nucleotides ) was determined in this way . Particular genes are located in the sequence by comparing the nucleotide sequence to that ex- pected from ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses