Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
From inside the book
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Page 20
... particular genes out of a pile of DNA frag- ments . Instead , we use bacteria . Bacteria can incorporate small ... particular gene is not easy ; the gene cloner faces a problem of numbers . First , the gene being sought may be rare ; it ...
... particular genes out of a pile of DNA frag- ments . Instead , we use bacteria . Bacteria can incorporate small ... particular gene is not easy ; the gene cloner faces a problem of numbers . First , the gene being sought may be rare ; it ...
Page 45
... particular transfer RNA also bind to the AUG ( or in some instances GUG ) codon on the mRNA . This transfer RNA ... particular amino acid and a particular transfer RNA have attached to a particular ( a ) Amino acid Enzyme Enzyme - amino ...
... particular transfer RNA also bind to the AUG ( or in some instances GUG ) codon on the mRNA . This transfer RNA ... particular amino acid and a particular transfer RNA have attached to a particular ( a ) Amino acid Enzyme Enzyme - amino ...
Page 119
... particular RFLPs that tend to be inherited with a particular disease . Generally the fragment differences are not due to a restriction site being created or disrupted by the diseased state itself ( sickle - cell anemia is an exception ) ...
... particular RFLPs that tend to be inherited with a particular disease . Generally the fragment differences are not due to a restriction site being created or disrupted by the diseased state itself ( sickle - cell anemia is an exception ) ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses