Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 97
... phages have genes that produce an RNA polymerase , so they do not have to rely on the host polymerase to make messenger RNA from phage genes . Many phages also have genes for their own DNA replication ma- chinery . When this apparatus ...
... phages have genes that produce an RNA polymerase , so they do not have to rely on the host polymerase to make messenger RNA from phage genes . Many phages also have genes for their own DNA replication ma- chinery . When this apparatus ...
Page 99
... phage particle . If a DNA fragment is inserted into a phage DNA molecule without destroying important phage genes , the phage will reproduce the frag- ment along with its own DNA when it infects a bacterial cell . Gene cloners can ...
... phage particle . If a DNA fragment is inserted into a phage DNA molecule without destroying important phage genes , the phage will reproduce the frag- ment along with its own DNA when it infects a bacterial cell . Gene cloners can ...
Page 100
... phage particles was placed in a plastic tube , mixed with ce- sium chloride , and centrifuged at 25,000 rpm for one day . During the centrifu- gation the phage particles formed a band ... Phage : DNA Bacterial DNA d . 100 Plasmids and Phages.
... phage particles was placed in a plastic tube , mixed with ce- sium chloride , and centrifuged at 25,000 rpm for one day . During the centrifu- gation the phage particles formed a band ... Phage : DNA Bacterial DNA d . 100 Plasmids and Phages.
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses