Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 97
... particles to seek new hosts . Phage efficiency is awesome . One phage can produce hundreds of progeny particles . Each progeny particle can infect a bacterial cell and produce many ... Phage plaque Bacterial cells ( b ) Bacteriophages 97.
... particles to seek new hosts . Phage efficiency is awesome . One phage can produce hundreds of progeny particles . Each progeny particle can infect a bacterial cell and produce many ... Phage plaque Bacterial cells ( b ) Bacteriophages 97.
Page 100
... particles was placed in a plastic tube , mixed with ce- sium chloride , and centrifuged at 25,000 rpm for one day . During the centrifu- gation the phage ... Phage : DNA Bacterial DNA d . 100 Plasmids and Phages.
... particles was placed in a plastic tube , mixed with ce- sium chloride , and centrifuged at 25,000 rpm for one day . During the centrifu- gation the phage ... Phage : DNA Bacterial DNA d . 100 Plasmids and Phages.
Page 129
... phage particles from each other . Phage plaques formed in the lawn of bacteria as each phage reproduced ( Figure 6-5 ) . Every plaque arose from a different phage particle , and each plaque contained millions of identical phage particles ...
... phage particles from each other . Phage plaques formed in the lawn of bacteria as each phage reproduced ( Figure 6-5 ) . Every plaque arose from a different phage particle , and each plaque contained millions of identical phage particles ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses