Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 80
... Primer 2 + Primer 1 Strand 2 DNA polymerase + nucleotide triphosphates Strand 1 + New DNA strands Strand 2 + Heat Strand 1 + Strand 2 Primer 1 Primer 2 Cool Figure 5-11 Amplification of DNA . ( a ) A double - stranded DNA molecule that ...
... Primer 2 + Primer 1 Strand 2 DNA polymerase + nucleotide triphosphates Strand 1 + New DNA strands Strand 2 + Heat Strand 1 + Strand 2 Primer 1 Primer 2 Cool Figure 5-11 Amplification of DNA . ( a ) A double - stranded DNA molecule that ...
Page 146
... primer to start synthesis of DNA ; thus , its synthesis begins only where the primer is located ) . DNA polymerase soon crosses into the cloned DNA region and uses it as a template to make new DNA . The polymerase can be stopped by ...
... primer to start synthesis of DNA ; thus , its synthesis begins only where the primer is located ) . DNA polymerase soon crosses into the cloned DNA region and uses it as a template to make new DNA . The polymerase can be stopped by ...
Page 197
... Primer Primer Reverse transcriptase action U3 R U5 ( b ) Reverse transcriptase then synthesizes a short DNA strand from this primer . ( c ) RNase H action degrades regions of RNA hybridized to DNA . ( d ) Strand transfer occurs by ...
... Primer Primer Reverse transcriptase action U3 R U5 ( b ) Reverse transcriptase then synthesizes a short DNA strand from this primer . ( c ) RNase H action degrades regions of RNA hybridized to DNA . ( d ) Strand transfer occurs by ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses