Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 97
... produce an RNA polymerase , so they do not have to rely on the host polymerase to make messenger RNA from phage genes . Many phages also have genes for their own DNA replication ma- chinery . When this apparatus is in place , the phages ...
... produce an RNA polymerase , so they do not have to rely on the host polymerase to make messenger RNA from phage genes . Many phages also have genes for their own DNA replication ma- chinery . When this apparatus is in place , the phages ...
Page 173
... produces anti- body molecules that are slightly different from those produced by other B lymphocytes . At different times a cell can produce antibodies that differ in their heavy chain constant regions . Thus the antibody can behave ...
... produces anti- body molecules that are slightly different from those produced by other B lymphocytes . At different times a cell can produce antibodies that differ in their heavy chain constant regions . Thus the antibody can behave ...
Page 219
... producing cells . When fused to a B cell , a myeloma cell stimulates the B cell to secrete large amounts of the antibody that the B - cell had been programmed to produce . The myeloma cell also immortalizes the B cell ( cultured B cells ...
... producing cells . When fused to a B cell , a myeloma cell stimulates the B cell to secrete large amounts of the antibody that the B - cell had been programmed to produce . The myeloma cell also immortalizes the B cell ( cultured B cells ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses