Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
From inside the book
Results 1-3 of 33
Page 71
... purified . To be useful for gene cloning , enzymes must be removed from living cells . The first step of purification is to obtain a large batch of cells , about a pound of cells in the case of bacteria . Then the cell walls must be ...
... purified . To be useful for gene cloning , enzymes must be removed from living cells . The first step of purification is to obtain a large batch of cells , about a pound of cells in the case of bacteria . Then the cell walls must be ...
Page 99
... purified by den- sity gradient centrifugation in a way similar to that described earlier for purification of plasmid DNA . Since DNA and protein have differ- ent densities , phages , which are a combination of DNA and protein , will ...
... purified by den- sity gradient centrifugation in a way similar to that described earlier for purification of plasmid DNA . Since DNA and protein have differ- ent densities , phages , which are a combination of DNA and protein , will ...
Page 145
... Purify DNA ( c ) Single - strand recombinant DNA { 0 M13 DNA Figure 9-3 Cloning into Phage M13 . ( a ) A restriction endonuclease is used to cut a human DNA fragment and a purified double - stranded replicative inter- mediate of phage ...
... Purify DNA ( c ) Single - strand recombinant DNA { 0 M13 DNA Figure 9-3 Cloning into Phage M13 . ( a ) A restriction endonuclease is used to cut a human DNA fragment and a purified double - stranded replicative inter- mediate of phage ...
Other editions - View all
Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses