Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 146
... region ( DNA polymerase requires a primer to start synthesis of DNA ; thus , its synthesis begins only where the primer is located ) . DNA polymerase soon crosses into the cloned DNA region and uses it as a template to make new DNA ...
... region ( DNA polymerase requires a primer to start synthesis of DNA ; thus , its synthesis begins only where the primer is located ) . DNA polymerase soon crosses into the cloned DNA region and uses it as a template to make new DNA ...
Page 215
... DNA Once a piece of DNA has been cloned , determining the sequence is straightforward . Often molecular biologists ... region as a probe for locating adjacent , overlap- ping regions in a collection of recombinant DNAs ( Figure AII - 1 ) ...
... DNA Once a piece of DNA has been cloned , determining the sequence is straightforward . Often molecular biologists ... region as a probe for locating adjacent , overlap- ping regions in a collection of recombinant DNAs ( Figure AII - 1 ) ...
Page 217
... Region I - plasmid recombinant DNA is cut by endonuclease R and X to liber- ate the short piece of DNA ( solid ) that represents the overlap between regions I and II . ( 3 ) The overlapping DNA piece is separated from all other pieces ...
... Region I - plasmid recombinant DNA is cut by endonuclease R and X to liber- ate the short piece of DNA ( solid ) that represents the overlap between regions I and II . ( 3 ) The overlapping DNA piece is separated from all other pieces ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses