Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 120
... endonuclease cleavage sites , one at each junction between human and plasmid DNA . ) ( 3 ) Cleavage of the recombinant plasmid DNA with restriction ... restriction Direction of DNA movement restriction 120 Gene Manipulation.
... endonuclease cleavage sites , one at each junction between human and plasmid DNA . ) ( 3 ) Cleavage of the recombinant plasmid DNA with restriction ... restriction Direction of DNA movement restriction 120 Gene Manipulation.
Page 121
... restriction map of the plasmid into which it has been. Restriction endonuclease cleavage site O 4 endonuclease Cut , splice Recombinant DNA molecules ( many types ) 2 Human DNA Gene cloning Restriction endonuclease cleavage site ...
... restriction map of the plasmid into which it has been. Restriction endonuclease cleavage site O 4 endonuclease Cut , splice Recombinant DNA molecules ( many types ) 2 Human DNA Gene cloning Restriction endonuclease cleavage site ...
Page 143
... Restriction endonuclease + Restriction endonuclease + WIL ( b ) ( c ) חד MIT யட Ligate Mix + Restriction endonuclease חד חחד חד M m WIL www UIL Figure 9-1 Regeneration of a Restriction Site . ( a ) Two DNA molecules con- taining a ...
... Restriction endonuclease + Restriction endonuclease + WIL ( b ) ( c ) חד MIT யட Ligate Mix + Restriction endonuclease חד חחד חד M m WIL www UIL Figure 9-1 Regeneration of a Restriction Site . ( a ) Two DNA molecules con- taining a ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses