Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 106
... splicing " creates new genetic arrangements . Like the film editor , a gene cloner needs two tools : scissors and splicing tape . The scissors biologists use are specific enzymes that obey an important rule : they cut only at specific ...
... splicing " creates new genetic arrangements . Like the film editor , a gene cloner needs two tools : scissors and splicing tape . The scissors biologists use are specific enzymes that obey an important rule : they cut only at specific ...
Page 165
... SPLICING Once genes had been purified , it was a straightforward process to de- termine the nucleotide sequences of the genes and of the DNA sur- rounding them . This led to the astounding discovery that in higher or- ganisms the coding ...
... SPLICING Once genes had been purified , it was a straightforward process to de- termine the nucleotide sequences of the genes and of the DNA sur- rounding them . This led to the astounding discovery that in higher or- ganisms the coding ...
Page 167
... splicing occurs during the processing , and exci- sion of a particular intron was found to occur even after all proteins had been removed . The intron RNA is self - splicing . After excision , the intron retains its splicing capability ...
... splicing occurs during the processing , and exci- sion of a particular intron was found to occur even after all proteins had been removed . The intron RNA is self - splicing . After excision , the intron retains its splicing capability ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses