Manual of Methods for General Bacteriology |
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Page 139
... Adjust pH to 3.5 to 4.0 with sterile H2SO4 . Trace metal solution : Ethylenediaminetetraacetic acid ( EDTA ) ZnSO4.7H2O CaCl2 MnCl2-4H2O FeSO4-7H2O ( NH4 ) 6M07024-4H2O CuSO4.5H2O CoCl2.6H2O Distilled water Adjust to pH 6.0 with KOH ...
... Adjust pH to 3.5 to 4.0 with sterile H2SO4 . Trace metal solution : Ethylenediaminetetraacetic acid ( EDTA ) ZnSO4.7H2O CaCl2 MnCl2-4H2O FeSO4-7H2O ( NH4 ) 6M07024-4H2O CuSO4.5H2O CoCl2.6H2O Distilled water Adjust to pH 6.0 with KOH ...
Page 432
... adjust pH to 6.8 with KOH . Sterilize by autoclaving . Note : Some strains of Azospirillum may require addition of 0.005 % yeast extract as a source of vitamins . 20.3.5 . Medium B ( 51 ) Pancreatic digest of casein USP Peptic digest of ...
... adjust pH to 6.8 with KOH . Sterilize by autoclaving . Note : Some strains of Azospirillum may require addition of 0.005 % yeast extract as a source of vitamins . 20.3.5 . Medium B ( 51 ) Pancreatic digest of casein USP Peptic digest of ...
Page 435
... adjust the pH to 6.8 . 20.3.19 . Leifson Modified O - F ( MOF ) Me- dium ( 55 ) 20.3.22 . Milk Medium , Prereduced ( 7 ) Fresh skim milk 100 ml 0.4 ml 1.0 ml 0.02 ml Resazurin solution , 0.025 % Hemin solution ( see 20.3.28 ) Vitamin K ...
... adjust the pH to 6.8 . 20.3.19 . Leifson Modified O - F ( MOF ) Me- dium ( 55 ) 20.3.22 . Milk Medium , Prereduced ( 7 ) Fresh skim milk 100 ml 0.4 ml 1.0 ml 0.02 ml Resazurin solution , 0.025 % Hemin solution ( see 20.3.28 ) Vitamin K ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes