Manual of Methods for General Bacteriology |
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Page 357
... Dissolve 50 mg of MnSO4 H2O in 100 ml of distilled NH1 * -free water . Hypochlorite reagent : Add 10 ml of commercial bleach ( 5 % sodium hypochlorite solution ) to 40 ml of NH * - free distilled water . Adjust the pH to 6.5 to 7.0 ...
... Dissolve 50 mg of MnSO4 H2O in 100 ml of distilled NH1 * -free water . Hypochlorite reagent : Add 10 ml of commercial bleach ( 5 % sodium hypochlorite solution ) to 40 ml of NH * - free distilled water . Adjust the pH to 6.5 to 7.0 ...
Page 436
... dissolved , add 200 ml of distilled water . Hemin solution : Dissolve 0.05 g of hemin in 1 ml of 1 N NaOH . Dilute to 100 ml with distilled water . Autoclave at 121 ° C for 15 min . Vitamin K solution : Dissolve 0.15 ml of vitamin K1 in ...
... dissolved , add 200 ml of distilled water . Hemin solution : Dissolve 0.05 g of hemin in 1 ml of 1 N NaOH . Dilute to 100 ml with distilled water . Autoclave at 121 ° C for 15 min . Vitamin K solution : Dissolve 0.15 ml of vitamin K1 in ...
Page 439
... Dissolve by gentle heating in a fume hood . Solution B : Sulfanilic acid HCl , 1.5 N solution Dissolve by gentle heating . 1.0 g 100 ml Mix equal volumes of solutions A and B just before use to make the test reagent . For Method 2 ...
... Dissolve by gentle heating in a fume hood . Solution B : Sulfanilic acid HCl , 1.5 N solution Dissolve by gentle heating . 1.0 g 100 ml Mix equal volumes of solutions A and B just before use to make the test reagent . For Method 2 ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes