Manual of Methods for General Bacteriology |
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Page 114
... incubate enrichment cultures at 0 to 5 ° C before attempting isolation . For instance , Inoue ( 31 ) was able to isolate psychrophilic bacteria from Antarctic soil by spreading dilutions over plates of a glucose - yeast extract ...
... incubate enrichment cultures at 0 to 5 ° C before attempting isolation . For instance , Inoue ( 31 ) was able to isolate psychrophilic bacteria from Antarctic soil by spreading dilutions over plates of a glucose - yeast extract ...
Page 122
... Incubate at 25 ° C for 1 to 2 days . The Formalin will kill contaminating bacteria but not Galli- onella . Transfer 1 - ml portions of the Formalin- treated culture to fresh bottles of medium with- out Formalin . Incubate cultures at 25 ...
... Incubate at 25 ° C for 1 to 2 days . The Formalin will kill contaminating bacteria but not Galli- onella . Transfer 1 - ml portions of the Formalin- treated culture to fresh bottles of medium with- out Formalin . Incubate cultures at 25 ...
Page 124
... Incubate at 30 ° C with agitation . Observe the culture micro- scopically at periodic intervals for the develop- ment of large , ovoid cells , 2 μm or more in diameter . Prepare a secondary enrichment cul- ture ; then purify by ...
... Incubate at 30 ° C with agitation . Observe the culture micro- scopically at periodic intervals for the develop- ment of large , ovoid cells , 2 μm or more in diameter . Prepare a secondary enrichment cul- ture ; then purify by ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes