Manual of Methods for General Bacteriology |
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Page 129
... Pasteur pipette . Another disadvantage is that the bacteria to be isolated must be able to withstand temporarily the 45 to A 50 ° C temperature of the molten agar . Thiamine p - Aminobenzoic acid Panthothenic acid Pyridoxamine HCl ...
... Pasteur pipette . Another disadvantage is that the bacteria to be isolated must be able to withstand temporarily the 45 to A 50 ° C temperature of the molten agar . Thiamine p - Aminobenzoic acid Panthothenic acid Pyridoxamine HCl ...
Page 183
... Pasteur pipette . 3. Carry out a final single dilution of the sam- ple in a ratio of at least 1 : 1 with 0.1 N HCl . This kills the bacteria and gives their surface a net positive charge so they will not aggregate but will adsorb onto ...
... Pasteur pipette . 3. Carry out a final single dilution of the sam- ple in a ratio of at least 1 : 1 with 0.1 N HCl . This kills the bacteria and gives their surface a net positive charge so they will not aggregate but will adsorb onto ...
Page 341
... Pasteur pipette plugged with glass wool in the constricted end . Tap the Pasteur pipette until the silica gel forms a layer on the glass wool . Rinse the Pasteur pipette and silica gel with 2 ml of chloroform- methanol ( 1 : 1 , vol ...
... Pasteur pipette plugged with glass wool in the constricted end . Tap the Pasteur pipette until the silica gel forms a layer on the glass wool . Rinse the Pasteur pipette and silica gel with 2 ml of chloroform- methanol ( 1 : 1 , vol ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes