Manual of Methods for General Bacteriology |
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Page 129
129 them out with a sterile instrument or punching them out with a sterile Pasteur
pipette . Another disadvantage is that the bacteria to be isolated must be able to
withstand temporarily the 45 to 50°C temperature of the molten agar .
129 them out with a sterile instrument or punching them out with a sterile Pasteur
pipette . Another disadvantage is that the bacteria to be isolated must be able to
withstand temporarily the 45 to 50°C temperature of the molten agar .
Page 334
Pipette the samples to be analyzed into thickwalled Pyrex test tubes . Adjust the
volume of all samples ... manufacturer ' s instructions . Add 1 drop of 4 N HCl with
a Pasteur pipette , and read the absorbance at 400 nm in glass cuvettes ( 16 . 1 .
Pipette the samples to be analyzed into thickwalled Pyrex test tubes . Adjust the
volume of all samples ... manufacturer ' s instructions . Add 1 drop of 4 N HCl with
a Pasteur pipette , and read the absorbance at 400 nm in glass cuvettes ( 16 . 1 .
Page 341
Transfer all the silica gel from each spot to a separate Pasteur pipette plugged
with glass wool in the constricted end . Tap the Pasteur pipette until the silica gel
forms a layer on the glass wool . Rinse the Pasteur pipette and silica gel with 2 ml
...
Transfer all the silica gel from each spot to a separate Pasteur pipette plugged
with glass wool in the constricted end . Tap the Pasteur pipette until the silica gel
forms a layer on the glass wool . Rinse the Pasteur pipette and silica gel with 2 ml
...
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Contents
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
GROWTH RALPH N COSTILOW Editor | 63 |
Copyright | |
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Common terms and phrases
absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter Microbiol microscopy mixture mutants needed obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York
Bibliographic information
| Title | Manual of Methods for General Bacteriology |
| Authors | Philipp Gerhardt, American Society for Microbiology |
| Editor | Philipp Gerhardt |
| Edition | illustrated |
| Publisher | American Society for Microbiology, 1981 |
| Original from | the University of Michigan |
| Digitized | 17 Jul 2008 |
| ISBN | 0914826301, 9780914826309 |
| Length | 524 pages |
| Subjects | › Science / General |
| Export Citation | BiBTeX EndNote RefMan |