Manual of Methods for General Bacteriology |
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Page 31
... Wash the slide several times in a solution consisting of 2 g of potassium metabisulfite and 5 ml of concentrated hydrochloric acid in 500 ml of distilled water . 8. Wash the slide with tap water , and counter- stain with a 0.002 % ( wt ...
... Wash the slide several times in a solution consisting of 2 g of potassium metabisulfite and 5 ml of concentrated hydrochloric acid in 500 ml of distilled water . 8. Wash the slide with tap water , and counter- stain with a 0.002 % ( wt ...
Page 331
... Wash the filter in a hood with 2- to 5 - ml volumes of ethanol - diethyl ether ( 1 : 1 , vol / vol ) warmed to 45 ° C in a water bath by allowing the solvents to pass slowly through the filter ( 10 min ) . Collect the filtrate in a test ...
... Wash the filter in a hood with 2- to 5 - ml volumes of ethanol - diethyl ether ( 1 : 1 , vol / vol ) warmed to 45 ° C in a water bath by allowing the solvents to pass slowly through the filter ( 10 min ) . Collect the filtrate in a test ...
Page 394
... Washing of cells . After harvest , it is generally necessary to wash the cells before they can be used for permeability assays . Usually , a single wash with a large vol- ume of fluid is sufficient to nearly rid the cells of medium ...
... Washing of cells . After harvest , it is generally necessary to wash the cells before they can be used for permeability assays . Usually , a single wash with a large vol- ume of fluid is sufficient to nearly rid the cells of medium ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes