Manual of Methods for General Bacteriology |
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Page 120
0375 % , phosphate buffer to neutralize the action of the as in cystine tellurite
blood agar ( 8 . 5 . 16 ) . Colosodium hydroxide . Centrifuge at 1 , 800 to 2 , 400
nies of corynebacteria are gray or black as a xg for 15 min to concentrate the ...
0375 % , phosphate buffer to neutralize the action of the as in cystine tellurite
blood agar ( 8 . 5 . 16 ) . Colosodium hydroxide . Centrifuge at 1 , 800 to 2 , 400
nies of corynebacteria are gray or black as a xg for 15 min to concentrate the ...
Page 132
Boil to dissolve agar . Sterilize Ferric citrate . . . 0 . 5 g at 121°C for 15 min .
Distilled water . . . . . . . . . . . . . . . . 100 ml 8 . 5 . 13 . Brilliant Green Lactose Bile
Broth Combine the three solutions and heat to 100°C ( available from BBL or
Difco as ...
Boil to dissolve agar . Sterilize Ferric citrate . . . 0 . 5 g at 121°C for 15 min .
Distilled water . . . . . . . . . . . . . . . . 100 ml 8 . 5 . 13 . Brilliant Green Lactose Bile
Broth Combine the three solutions and heat to 100°C ( available from BBL or
Difco as ...
Page 143
149 Media prepared in the solid state , in the form For further information about
the chemical comof firm gels , have been used in bacteriology since position and
structure of agar , see references 4 adopted by Robert Koch . The most important
...
149 Media prepared in the solid state , in the form For further information about
the chemical comof firm gels , have been used in bacteriology since position and
structure of agar , see references 4 adopted by Robert Koch . The most important
...
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Contents
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
GROWTH RALPH N COSTILOW Editor | 63 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter Microbiol microscopy mixture mutants needed obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York