Manual of Methods for General Bacteriology |
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Page 102
Biotin requirement is satisfied by biocytin or dethiobiotin or by 1 , 000 times the
amount of Tween 80 or sodium oleate . • Add ( per liter ) : nicotinic acid , 5 mg ;
calcium pantothenate , 1 mg ; pyridoxine , 1 mg ; biotin , 100 ug ; and folinic acid
...
Biotin requirement is satisfied by biocytin or dethiobiotin or by 1 , 000 times the
amount of Tween 80 or sodium oleate . • Add ( per liter ) : nicotinic acid , 5 mg ;
calcium pantothenate , 1 mg ; pyridoxine , 1 mg ; biotin , 100 ug ; and folinic acid
...
Page 311
Then determination of ' H in the isolated material gives the amount of sterol
originally present . For this , only the specific activity of the acetic anhydride is
needed ; i . e . : cpm recovered sterol recovery =cpm added amount of sterol (
umol ) ...
Then determination of ' H in the isolated material gives the amount of sterol
originally present . For this , only the specific activity of the acetic anhydride is
needed ; i . e . : cpm recovered sterol recovery =cpm added amount of sterol (
umol ) ...
Page 464
Add trichloroacetic acid the 10 - ul amounts . Keep the temperature con - to a final
concentration of 5 % , and again mix stant during dispensing to avoid the effect of
well on a Vortex mixer . Cool in a refrigerator for temperature and sample size ...
Add trichloroacetic acid the 10 - ul amounts . Keep the temperature con - to a final
concentration of 5 % , and again mix stant during dispensing to avoid the effect of
well on a Vortex mixer . Cool in a refrigerator for temperature and sample size ...
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Contents
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
GROWTH RALPH N COSTILOW Editor | 63 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter Microbiol microscopy mixture mutants needed obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York