Manual of Methods for General Bacteriology |
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Page 46
... antisera are commercially available . A number of treatises on theory and practice have been published ( 83-85 ) . The principle of the fluorescent - antibody method has been adapted to electron micros- copy by coupling antibodies or ...
... antisera are commercially available . A number of treatises on theory and practice have been published ( 83-85 ) . The principle of the fluorescent - antibody method has been adapted to electron micros- copy by coupling antibodies or ...
Page 430
... antiserum according to the manufacturer's di- rection . Place a drop of the antiserum into one of the rings , and in another ring place a drop of saline ( 0.85 % NaCl ) or normal rabbit serum . Mix FIG . 4. Diagram showing how the end ...
... antiserum according to the manufacturer's di- rection . Place a drop of the antiserum into one of the rings , and in another ring place a drop of saline ( 0.85 % NaCl ) or normal rabbit serum . Mix FIG . 4. Diagram showing how the end ...
Page 432
... antiserum should be prepared fresh daily . The undiluted stock antiserum is stable when stored in a refrigerator below 8 ° C . 20.3 . RECIPES FOR MEDIA 20.3.1 Medium A ( 51 ) Peptone Glycerol K2SO4 ( anhydrous ) 20.0 g 10.0 ml 10.0 g ...
... antiserum should be prepared fresh daily . The undiluted stock antiserum is stable when stored in a refrigerator below 8 ° C . 20.3 . RECIPES FOR MEDIA 20.3.1 Medium A ( 51 ) Peptone Glycerol K2SO4 ( anhydrous ) 20.0 g 10.0 ml 10.0 g ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes