Manual of Methods for General Bacteriology |
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Page 18
... appropriate state by low - power ( 10 × objective ) microscopy during growth . If preparations must be made from fluid media , transfer an appropriate amount of growth to an area on a well - dried agar medium and allow the fluid to sink ...
... appropriate state by low - power ( 10 × objective ) microscopy during growth . If preparations must be made from fluid media , transfer an appropriate amount of growth to an area on a well - dried agar medium and allow the fluid to sink ...
Page 68
... appropriate solution as determined with a pH meter . To prepare about 100 ml of a phosphate buffer at pH 6.0 , titrate 95 ml of the monobasic solution with the dibasic solution . The most commonly used buffers in bacterial growth media ...
... appropriate solution as determined with a pH meter . To prepare about 100 ml of a phosphate buffer at pH 6.0 , titrate 95 ml of the monobasic solution with the dibasic solution . The most commonly used buffers in bacterial growth media ...
Page 367
... appropriate environment containing chlor- amphenicol ( ca. 50 to 100 μg / ml ) to inhibit pro- tein synthesis . In some experiments , such as those concerned with transport analyses , it is often advisable that the cells contain ...
... appropriate environment containing chlor- amphenicol ( ca. 50 to 100 μg / ml ) to inhibit pro- tein synthesis . In some experiments , such as those concerned with transport analyses , it is often advisable that the cells contain ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes