Manual of Methods for General Bacteriology |
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Page 102
This provides the washed suspension of cells used to inoculate the assay tubes . The assay is carried out in 18 by 150 mm lipless culture tubes individually supported in a metal rack . When growth response is based on acid production ...
This provides the washed suspension of cells used to inoculate the assay tubes . The assay is carried out in 18 by 150 mm lipless culture tubes individually supported in a metal rack . When growth response is based on acid production ...
Page 376
Use a blank lacking the Lprior to assay for B - galactosidase . Just prior to threonine with each set of assays . Incubate the assay , they are removed from the ice bath and tubes at 37 ° C for a predetermined period ( ca.
Use a blank lacking the Lprior to assay for B - galactosidase . Just prior to threonine with each set of assays . Incubate the assay , they are removed from the ice bath and tubes at 37 ° C for a predetermined period ( ca.
Page 377
of the assay . Commercially available L - ribulose amount of glutamine synthetase present , since o - nitrophenylhydrazone is used as such to con- both the adenylylated and deadenylylated forms struct a standard curve ( range of 0 to ...
of the assay . Commercially available L - ribulose amount of glutamine synthetase present , since o - nitrophenylhydrazone is used as such to con- both the adenylylated and deadenylylated forms struct a standard curve ( range of 0 to ...
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Contents
1 | 22 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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Common terms and phrases
absorbance acid activity added addition Adjust agar allow amino amount anaerobic applications appropriate assay autoclaving bacteria base broth buffer cells centrifuge chemical colonies column components concentration containing counting cover culture cytoplasmic described determine dilution Dissolve distilled water drop effective electron enzyme examination example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method microscopy mixture mutants needed objective obtained organisms oxygen plasmid plate positive possible prepared present Press procedure protein reaction reagent references remove sample selection separation slide sodium solution specific specimen staining standard sterile surface suspension Table techniques temperature tion transfer tube usually volume Wash York