Manual of Methods for General Bacteriology |
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Page 102
... assay tubes . The assay is carried out in 18 by 150 mm lipless culture tubes individually supported in a metal rack . When growth response is based on acid production , the total volume may be as low as 2 ml , as recommended by Steele ...
... assay tubes . The assay is carried out in 18 by 150 mm lipless culture tubes individually supported in a metal rack . When growth response is based on acid production , the total volume may be as low as 2 ml , as recommended by Steele ...
Page 376
... assay for B - galactosidase . Just prior to assay , they are removed from the ice bath and treated with 1 drop of toluene and 1 drop of 0.1 % sodium deoxycholate , stoppered , shaken at 37 ° C for 15 min , and then put back in the ice ...
... assay for B - galactosidase . Just prior to assay , they are removed from the ice bath and treated with 1 drop of toluene and 1 drop of 0.1 % sodium deoxycholate , stoppered , shaken at 37 ° C for 15 min , and then put back in the ice ...
Page 377
... assay for kinases described above involving NADH * oxidation : glutamate + NADH✦ + NH3 + + phosphoenolpyruvate pyruvate kinase lactate dehydrogenase glutamine synthetase glutamine + NAD + lactate + inorganic phosphate Although ATP is ...
... assay for kinases described above involving NADH * oxidation : glutamate + NADH✦ + NH3 + + phosphoenolpyruvate pyruvate kinase lactate dehydrogenase glutamine synthetase glutamine + NAD + lactate + inorganic phosphate Although ATP is ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes