Manual of Methods for General Bacteriology |
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Page 81
Biotin is stable to autoclaving and to acids , but it is readily oxidized to the
sulfoxide and sulfone . These oxidation products also promote growth of certain
bacteria , but only if they can convert them to biotin . Oxidation is a problem only
in ...
Biotin is stable to autoclaving and to acids , but it is readily oxidized to the
sulfoxide and sulfone . These oxidation products also promote growth of certain
bacteria , but only if they can convert them to biotin . Oxidation is a problem only
in ...
Page 164
However , most simple media for cultivation of bacteria can be autoclaved prior to
use , but larger volumes of medium ... Take special care to ensure establishment
and maintenance of the chemically reduced state when autoclaving large ...
However , most simple media for cultivation of bacteria can be autoclaved prior to
use , but larger volumes of medium ... Take special care to ensure establishment
and maintenance of the chemically reduced state when autoclaving large ...
Page 435
Dispense into 13 by 100 mm tubes , and sterilize by autoclaving . Dissolve
ingredients in the distilled water , adjust the pH to 7 . 5 , and sterilize by
autoclaving . Autoclave the seawater separately . After cooling to 45 to 50°C , mix
the two ...
Dispense into 13 by 100 mm tubes , and sterilize by autoclaving . Dissolve
ingredients in the distilled water , adjust the pH to 7 . 5 , and sterilize by
autoclaving . Autoclave the seawater separately . After cooling to 45 to 50°C , mix
the two ...
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Contents
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
GROWTH RALPH N COSTILOW Editor | 63 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter Microbiol microscopy mixture mutants needed obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York