Manual of Methods for General Bacteriology |
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Page 413
... agar slant ( see 20.3.25 ) or other medium lacking blood . After incubation , trickle 1 ml of 3 % hydrogen peroxide down the slant . Examine immediately and after 5 min for the evolution of bubbles , which indicates a positive test ...
... agar slant ( see 20.3.25 ) or other medium lacking blood . After incubation , trickle 1 ml of 3 % hydrogen peroxide down the slant . Examine immediately and after 5 min for the evolution of bubbles , which indicates a positive test ...
Page 416
... blood agar ( 20.3.7 ) . Incubate at 37 ° C for 24 to 48 h . Beta ( B ) hemolysis : a clear , colorless zone surrounds the colonies . The erythrocytes in the zone are completely lysed . Alpha ( a ) hemolysis : an indis- tinct zone of ...
... blood agar ( 20.3.7 ) . Incubate at 37 ° C for 24 to 48 h . Beta ( B ) hemolysis : a clear , colorless zone surrounds the colonies . The erythrocytes in the zone are completely lysed . Alpha ( a ) hemolysis : an indis- tinct zone of ...
Page 432
... Agar Distilled water 0.026 g 0.017 g 0.002 g 3.58 g ( 5.00 g ) 5.0 ml 1.75 g 1,000 ml Combine gradients and adjust ... blood serum . Mix and dis- pense into plates . Note : 1 % dehydrated oxgall 10 % bile ( vol / vol ) ; to make " 40 % bile ...
... Agar Distilled water 0.026 g 0.017 g 0.002 g 3.58 g ( 5.00 g ) 5.0 ml 1.75 g 1,000 ml Combine gradients and adjust ... blood serum . Mix and dis- pense into plates . Note : 1 % dehydrated oxgall 10 % bile ( vol / vol ) ; to make " 40 % bile ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes