Manual of Methods for General Bacteriology |
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Page 114
... broth . Store the broths at 4 ° C for up to 3 months , and attempt isolation at weekly intervals for the first 4 ... broth suspension to 5 ml of KCNS - listeria broth ( add 3.75 % potassium thiocyanate to listeria broth prior to ...
... broth . Store the broths at 4 ° C for up to 3 months , and attempt isolation at weekly intervals for the first 4 ... broth suspension to 5 ml of KCNS - listeria broth ( add 3.75 % potassium thiocyanate to listeria broth prior to ...
Page 120
... broth ) . The high pH of the TCBS agar and the enrichment broth inhibit the growth of most contaminants . In the case of V. cholerae , incu- bate the enrichment broth for 6 to 8 h at 35 ° C ; then streak nonselective and selective agar ...
... broth ) . The high pH of the TCBS agar and the enrichment broth inhibit the growth of most contaminants . In the case of V. cholerae , incu- bate the enrichment broth for 6 to 8 h at 35 ° C ; then streak nonselective and selective agar ...
Page 172
... broth for further study by batch centrifugation may require discarding a portion of the broth to avoid resuspension of the cell pellet during removal of the supernatant . 10.4.1 . Filtration Cake filtration . Two types of filtration are ...
... broth for further study by batch centrifugation may require discarding a portion of the broth to avoid resuspension of the cell pellet during removal of the supernatant . 10.4.1 . Filtration Cake filtration . Two types of filtration are ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes