Manual of Methods for General Bacteriology |
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Page 59
... buffer systems , in which the upper buffer contains a rapidly moving ionic species which moves through the gel as a zone or front . Proteins are compressed by this moving front into a very thin band at the time they enter the running ...
... buffer systems , in which the upper buffer contains a rapidly moving ionic species which moves through the gel as a zone or front . Proteins are compressed by this moving front into a very thin band at the time they enter the running ...
Page 67
... buffer system in the medium . Consider the following in selecting a buffer to use : 1. The pH desired . 2. Possible inhibitory or toxic effects of the buffer . 3. Possible utilization of the buffer by the bacterial culture . 4. Possible ...
... buffer system in the medium . Consider the following in selecting a buffer to use : 1. The pH desired . 2. Possible inhibitory or toxic effects of the buffer . 3. Possible utilization of the buffer by the bacterial culture . 4. Possible ...
Page 68
... buffer at pH 6.0 , titrate 95 ml of the monobasic solution with the dibasic solution . The most commonly used buffers in bacterial growth media are phosphate , Tris - hydrochlo- ride , citrate , and acetate . These four systems will buffer ...
... buffer at pH 6.0 , titrate 95 ml of the monobasic solution with the dibasic solution . The most commonly used buffers in bacterial growth media are phosphate , Tris - hydrochlo- ride , citrate , and acetate . These four systems will buffer ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes