Manual of Methods for General Bacteriology |
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Page 53
... cells and empty " ghosts " or large cell envelope fragments . When procedures that involve conversion of cells to spheroplasts or protoplasts in osmotically stabilized medium are employed , it is essential to determine the per- centage ...
... cells and empty " ghosts " or large cell envelope fragments . When procedures that involve conversion of cells to spheroplasts or protoplasts in osmotically stabilized medium are employed , it is essential to determine the per- centage ...
Page 167
... cells that are in similar states of the cell growth cycle . The data of Kubitschek ( 34 ) and Poole ( 49 ) show that the cell volume of Escherichia coli increases linearly during the cell cycle while its cell mass increases ...
... cells that are in similar states of the cell growth cycle . The data of Kubitschek ( 34 ) and Poole ( 49 ) show that the cell volume of Escherichia coli increases linearly during the cell cycle while its cell mass increases ...
Page 505
... cells . The dispersion of cells can be maintained , or sometimes attained , by addi- tion to the diluent of a chemical agent that absorbs and imparts an electronegative repulsive charge to each cell . A detergent agent ( e.g. , 0.1 ...
... cells . The dispersion of cells can be maintained , or sometimes attained , by addi- tion to the diluent of a chemical agent that absorbs and imparts an electronegative repulsive charge to each cell . A detergent agent ( e.g. , 0.1 ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes