Manual of Methods for General Bacteriology |
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Page 174
It will probably be impossible An example of a centrifugation procedure is to prevent partial resuspension of the bacterial ... Prepare an appropriate diluent for washing to remain in the centrifuge tube with the cells . of the cells ...
It will probably be impossible An example of a centrifugation procedure is to prevent partial resuspension of the bacterial ... Prepare an appropriate diluent for washing to remain in the centrifuge tube with the cells . of the cells ...
Page 330
Centrifuge the radioactively labeled cells ( approximately 10 mg , dry weight ) from the growth medium ( 5,000 X g for ... and wash the sedimented cells twice by centrifugation with ice - cold ( 5 ° C ) 0.85 % saline ( onefifth of the ...
Centrifuge the radioactively labeled cells ( approximately 10 mg , dry weight ) from the growth medium ( 5,000 X g for ... and wash the sedimented cells twice by centrifugation with ice - cold ( 5 ° C ) 0.85 % saline ( onefifth of the ...
Page 347
Centrifuge indicates contamination by membrane , cell wall , the concentrate at 3,000 xg for 10 min to remove or cytoplasmic proteins . insoluble material . Discard the sediment . Half Good examples of approaches for a more com- the ...
Centrifuge indicates contamination by membrane , cell wall , the concentrate at 3,000 xg for 10 min to remove or cytoplasmic proteins . insoluble material . Discard the sediment . Half Good examples of approaches for a more com- the ...
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Contents
1 | 22 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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Common terms and phrases
absorbance acid activity added addition Adjust agar allow amino amount anaerobic applications appropriate assay autoclaving bacteria base broth buffer cells centrifuge chemical colonies column components concentration containing counting cover culture cytoplasmic described determine dilution Dissolve distilled water drop effective electron enzyme examination example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method microscopy mixture mutants needed objective obtained organisms oxygen plasmid plate positive possible prepared present Press procedure protein reaction reagent references remove sample selection separation slide sodium solution specific specimen staining standard sterile surface suspension Table techniques temperature tion transfer tube usually volume Wash York