Manual of Methods for General Bacteriology |
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Page 174
... centrifuge refrig- eration system to cool the head . If this is done , the centrifuge rotor will contract and seize the spindle , making it very difficult to remove at a later time . 3. Sterilize several capped centrifuge tubes ...
... centrifuge refrig- eration system to cool the head . If this is done , the centrifuge rotor will contract and seize the spindle , making it very difficult to remove at a later time . 3. Sterilize several capped centrifuge tubes ...
Page 330
... centrifuge : Capable of achieving 10,000 × g with a centrifuge head that holds 30 - ml centri- fuge tubes 17.1.2 . Labeling of Cells ( CAUTION : The use of radioactive isotopes is strictly regulated . All personnel involved in their use ...
... centrifuge : Capable of achieving 10,000 × g with a centrifuge head that holds 30 - ml centri- fuge tubes 17.1.2 . Labeling of Cells ( CAUTION : The use of radioactive isotopes is strictly regulated . All personnel involved in their use ...
Page 347
... Centrifuge the bacteria from approximately 3 liters of growth medium at 10,000 × g for 15 min . Suspend the sedimented cells in 300 ml of ice- cold saline solution , and centrifuge again . Repeat the resuspension and centrifugation to ...
... Centrifuge the bacteria from approximately 3 liters of growth medium at 10,000 × g for 15 min . Suspend the sedimented cells in 300 ml of ice- cold saline solution , and centrifuge again . Repeat the resuspension and centrifugation to ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes