Manual of Methods for General Bacteriology |
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Page 187
... colonies on all of the plates to learn how uniform your spreading tech- nique has become . Those that have a larger number of colonies are especially useful in this regard , even if they have too many colonies to count . Also note ...
... colonies on all of the plates to learn how uniform your spreading tech- nique has become . Those that have a larger number of colonies are especially useful in this regard , even if they have too many colonies to count . Also note ...
Page 232
... colonies can be distinguished from the nonmutants by a different color in , or adjacent to , the bacterial colonies . One example of this application , pre- sented below , involves the detection of lactose- nonfermenting mutants by ...
... colonies can be distinguished from the nonmutants by a different color in , or adjacent to , the bacterial colonies . One example of this application , pre- sented below , involves the detection of lactose- nonfermenting mutants by ...
Page 233
... colonies . Plates 2 and 3 then compared to identify the auxo- trophic mutant colonies FIG . 2. Replica plating method for screening auxotrophic mutants of a bacterium . 5. Pick the identified auxotrophic colonies with sterile toothpicks ...
... colonies . Plates 2 and 3 then compared to identify the auxo- trophic mutant colonies FIG . 2. Replica plating method for screening auxotrophic mutants of a bacterium . 5. Pick the identified auxotrophic colonies with sterile toothpicks ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes