Manual of Methods for General Bacteriology |
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Page 164
However , most simple media for cultivation of bacteria can be autoclaved prior to
use , but larger volumes of medium require longer autoclaving times . Vessels
containing approximately 10 liters of liquid should be autoclaved at 121°C for 30
...
However , most simple media for cultivation of bacteria can be autoclaved prior to
use , but larger volumes of medium require longer autoclaving times . Vessels
containing approximately 10 liters of liquid should be autoclaved at 121°C for 30
...
Page 212
Harvest broth cul - tures by aseptic centrifugation , and suspend the pellet with
sterile skim milk to yield a cell sus - pension containing at least 10° cells per ml .
Use the same procedure when employing sucrose as the cryoprotective agent .
Harvest broth cul - tures by aseptic centrifugation , and suspend the pellet with
sterile skim milk to yield a cell sus - pension containing at least 10° cells per ml .
Use the same procedure when employing sucrose as the cryoprotective agent .
Page 254
Add 200 ul of the adsorption mixture per 2 ml of L soft agar containing 2 . 5 mM
CaCl2 , and immediately distribute on an L agar + 2 . 5 mM CaCl2 plate . Prepare
five to eight plates in this way . ( Each plate will be seeded with 6 X 10° to 8 x 106
...
Add 200 ul of the adsorption mixture per 2 ml of L soft agar containing 2 . 5 mM
CaCl2 , and immediately distribute on an L agar + 2 . 5 mM CaCl2 plate . Prepare
five to eight plates in this way . ( Each plate will be seeded with 6 X 10° to 8 x 106
...
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Contents
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
GROWTH RALPH N COSTILOW Editor | 63 |
Copyright | |
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Common terms and phrases
absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter Microbiol microscopy mixture mutants needed obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York
Bibliographic information
| Title | Manual of Methods for General Bacteriology |
| Authors | Philipp Gerhardt, American Society for Microbiology |
| Editor | Philipp Gerhardt |
| Edition | illustrated, reprint |
| Publisher | American Society for Microbiology, 1981 |
| Original from | the University of Michigan |
| Digitized | 17 Jul 2008 |
| ISBN | 0914826301, 9780914826309 |
| Length | 524 pages |
| Subjects | › Science / General |
| Export Citation | BiBTeX EndNote RefMan |