Manual of Methods for General Bacteriology |
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Page 148
usually helpful to grow the inoculum for the agar when supplied with a source of fixed nitrogen in a broth containing a minimum concentration ( such as ammonium sulfate ) , but can only grow of the particular growth factor to be tested ...
usually helpful to grow the inoculum for the agar when supplied with a source of fixed nitrogen in a broth containing a minimum concentration ( such as ammonium sulfate ) , but can only grow of the particular growth factor to be tested ...
Page 164
Vessels containing approximately 10 liters of liquid should be autoclaved at 121 ° C for 30 to 90 min , depending upon ... containing solids such as cornmeal flour or soy flour may require up to 90 min for complete sterilization of a 10 ...
Vessels containing approximately 10 liters of liquid should be autoclaved at 121 ° C for 30 to 90 min , depending upon ... containing solids such as cornmeal flour or soy flour may require up to 90 min for complete sterilization of a 10 ...
Page 212
Harvest broth cultures by aseptic centrifugation , and suspend the pellet with sterile skim milk to yield a cell suspension containing at least 106 cells per ml . Use the same procedure when employing sucrose as the cryoprotective agent ...
Harvest broth cultures by aseptic centrifugation , and suspend the pellet with sterile skim milk to yield a cell suspension containing at least 106 cells per ml . Use the same procedure when employing sucrose as the cryoprotective agent ...
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Contents
1 | 22 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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Common terms and phrases
absorbance acid activity added addition Adjust agar allow amino amount anaerobic applications appropriate assay autoclaving bacteria base broth buffer cells centrifuge chemical colonies column components concentration containing counting cover culture cytoplasmic described determine dilution Dissolve distilled water drop effective electron enzyme examination example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method microscopy mixture mutants needed objective obtained organisms oxygen plasmid plate positive possible prepared present Press procedure protein reaction reagent references remove sample selection separation slide sodium solution specific specimen staining standard sterile surface suspension Table techniques temperature tion transfer tube usually volume Wash York
Bibliographic information
| Title | Manual of Methods for General Bacteriology |
| Authors | Philipp Gerhardt, American Society for Microbiology |
| Editor | Philipp Gerhardt |
| Edition | illustrated |
| Publisher | American Society for Microbiology, 1981 |
| Original from | the University of Michigan |
| Digitized | 17 Jul 2008 |
| ISBN | 0914826301, 9780914826309 |
| Length | 524 pages |
| Subjects | › Science / General |
| Export Citation | BiBTeX EndNote RefMan |