Manual of methods for general bacteriology: by Philipp Gerhardt, editor-in-chief ; R.G.E. Murray, editor, I. Morphology ... [et al.]. |
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Page 212
Harvest broth cultures by aseptic centrifugation, and suspend the pellet with
sterile skim milk to yield a cell suspension containing at least 108 cells per ml.
Use the same procedure when employing sucrose as the cryoprotective agent.
Harvest broth cultures by aseptic centrifugation, and suspend the pellet with
sterile skim milk to yield a cell suspension containing at least 108 cells per ml.
Use the same procedure when employing sucrose as the cryoprotective agent.
Page 254
Add 200 /d of the adsorption mixture per 2 ml of L soft agar containing 2.5 mM
CaCl2, and immediately distribute on an L agar + 2.5 mM CaCl2 plate. Prepare
five to eight plates in this way. (Each plate will be seeded with 6 x 105 to 8 x 105
PI ...
Add 200 /d of the adsorption mixture per 2 ml of L soft agar containing 2.5 mM
CaCl2, and immediately distribute on an L agar + 2.5 mM CaCl2 plate. Prepare
five to eight plates in this way. (Each plate will be seeded with 6 x 105 to 8 x 105
PI ...
Page 470
Heat the preparations for 5 min in a boiling- water bath, and after rapid cooling in
an ice bath, store the preparations at — 80°C. Immobilization of DNA and
preincubation of DNA-containing membranes. DNA is immobilized on
nitrocellulose ...
Heat the preparations for 5 min in a boiling- water bath, and after rapid cooling in
an ice bath, store the preparations at — 80°C. Immobilization of DNA and
preincubation of DNA-containing membranes. DNA is immobilized on
nitrocellulose ...
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Contents
Electron Microscopy Roger M CoLe anD Terry J Popkin | 34 |
Cell Fractionation CarL A Schnaitman | 52 |
GROWTH RaLph N CostiLow Editor | 65 |
Copyright | |
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Common terms and phrases
acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction g Distilled glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen Pasteur pipette pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove riboflavin salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vol/vol volume Wash wt/vol yeast extract
Bibliographic information
| Title | Manual of methods for general bacteriology: by Philipp Gerhardt, editor-in-chief ; R.G.E. Murray, editor, I. Morphology ... [et al.]. |
| Author | American Society for Microbiology |
| Editors | Philipp Gerhardt, American Society for Microbiology |
| Edition | illustrated, reprint |
| Publisher | American Society for Microbiology, 1981 |
| Original from | the University of Michigan |
| Digitized | 17 Jul 2008 |
| ISBN | 0914826301, 9780914826309 |
| Length | 524 pages |
| Subjects | › › Science / General Science / Life Sciences / Microbiology |
| Export Citation | BiBTeX EndNote RefMan |