Manual of Methods for General Bacteriology |
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Page 30
... Cytoplasmic Inclusions Many bacteria grown under certain cultural conditions produce , as a result of metabolic re- actions , deposits within the cytoplasm which are termed " inclusions . " Among these are deposits of fat , poly - ẞ ...
... Cytoplasmic Inclusions Many bacteria grown under certain cultural conditions produce , as a result of metabolic re- actions , deposits within the cytoplasm which are termed " inclusions . " Among these are deposits of fat , poly - ẞ ...
Page 60
... ( CYTOPLASM AND PERI- PLASM ) Centrifuge at 200,000 × g for 1 h SEDIMENT I SUPERNATANT III ( CYTOPLASMIC MEMBRANE ) Combine with Sedi- ment II SEDIMENT II Suspend combined sediments at 10 mg of protein per ml in same buffer used for ...
... ( CYTOPLASM AND PERI- PLASM ) Centrifuge at 200,000 × g for 1 h SEDIMENT I SUPERNATANT III ( CYTOPLASMIC MEMBRANE ) Combine with Sedi- ment II SEDIMENT II Suspend combined sediments at 10 mg of protein per ml in same buffer used for ...
Page 61
... cytoplasmic membrane . The " major " outer membrane proteins ( 1 ) visu- alized on SDS - polyacrylamide gels are useful markers for the outer membrane , as are specific lipopolysaccharide sugars or phospholipases ( 8 ) . We have ...
... cytoplasmic membrane . The " major " outer membrane proteins ( 1 ) visu- alized on SDS - polyacrylamide gels are useful markers for the outer membrane , as are specific lipopolysaccharide sugars or phospholipases ( 8 ) . We have ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes