Manual of Methods for General Bacteriology |
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Page 30
Leifson method for staining flagella . Poly - 3 - hydroxybutyrate inclusions appear as Solution 1 : blue - black droplets , and cytoplasmic parts of the Sodium chloride , 1.5 g organism appear pink . Distilled water , 100 ml Solution 2 ...
Leifson method for staining flagella . Poly - 3 - hydroxybutyrate inclusions appear as Solution 1 : blue - black droplets , and cytoplasmic parts of the Sodium chloride , 1.5 g organism appear pink . Distilled water , 100 ml Solution 2 ...
Page 60
This scheme takes advantage of the fact that , in the presence of low levels of Mg2 + ( contributed in this case by the cells themselves and the breakage buffer ) , the outer membrane is insoluble in Triton X - 100 while the cytoplasmic ...
This scheme takes advantage of the fact that , in the presence of low levels of Mg2 + ( contributed in this case by the cells themselves and the breakage buffer ) , the outer membrane is insoluble in Triton X - 100 while the cytoplasmic ...
Page 61
Cytochromes or succinic dehydrogenase are convenient markers for the cytoplasmic membrane . The " major ” outer membrane proteins ( 1 ) visualized on SDS - polyacrylamide gels are useful markers for the outer membrane , as are specific ...
Cytochromes or succinic dehydrogenase are convenient markers for the cytoplasmic membrane . The " major ” outer membrane proteins ( 1 ) visualized on SDS - polyacrylamide gels are useful markers for the outer membrane , as are specific ...
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Contents
1 | 22 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino amount anaerobic applications appropriate assay autoclaving bacteria base broth buffer cells centrifuge chemical colonies column components concentration containing counting cover culture cytoplasmic described determine dilution Dissolve distilled water drop effective electron enzyme examination example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method microscopy mixture mutants needed objective obtained organisms oxygen plasmid plate positive possible prepared present Press procedure protein reaction reagent references remove sample selection separation slide sodium solution specific specimen staining standard sterile surface suspension Table techniques temperature tion transfer tube usually volume Wash York