Manual of Methods for General Bacteriology |
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Page 225
... effective in curing cells of plasmids High Very effective in curing cells of plasmids Acridine dyes , ethidium bromide Novobiocin replication Blocks DNA replication into 5 - ml portions , and centrifuge for 5 min at 5,000 to 6,000 × g ...
... effective in curing cells of plasmids High Very effective in curing cells of plasmids Acridine dyes , ethidium bromide Novobiocin replication Blocks DNA replication into 5 - ml portions , and centrifuge for 5 min at 5,000 to 6,000 × g ...
Page 368
... effective disruption of bacteria ( and yeasts ) . Most of the other techniques have specialized uses or are effective only with orga- nisms ( such as protozoans or animal tissues ) which are easier to disrupt than are bacteria . Of the ...
... effective disruption of bacteria ( and yeasts ) . Most of the other techniques have specialized uses or are effective only with orga- nisms ( such as protozoans or animal tissues ) which are easier to disrupt than are bacteria . Of the ...
Page 369
... effective means for rupturing bacterial cells . A variety of sonicators are commercially available . Although relatively thick suspensions of cells can be used , breakage efficiency is decreased considerably in viscous solutions . As ...
... effective means for rupturing bacterial cells . A variety of sonicators are commercially available . Although relatively thick suspensions of cells can be used , breakage efficiency is decreased considerably in viscous solutions . As ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes