Manual of Methods for General Bacteriology |
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Page 27
... endospores . Ziehl - Neelsen method for acid - fast stain- ing . Carbolfuchsin stain : Basic fuchsin , 0.3 g Ethanol , 95 % ( vol / vol ) , 10 ml Phenol , heat - melted crystals , 5 ml Distilled water , 95 ml Dissolve the basic fuchsin ...
... endospores . Ziehl - Neelsen method for acid - fast stain- ing . Carbolfuchsin stain : Basic fuchsin , 0.3 g Ethanol , 95 % ( vol / vol ) , 10 ml Phenol , heat - melted crystals , 5 ml Distilled water , 95 ml Dissolve the basic fuchsin ...
Page 28
... Endospores Endospores of living bacteria , when viewed unstained , appear edged in black and shine brightly in a plane slightly above true focus . However , do not assume that any highly refrac- tile body within a bacterium is an endospore ...
... Endospores Endospores of living bacteria , when viewed unstained , appear edged in black and shine brightly in a plane slightly above true focus . However , do not assume that any highly refrac- tile body within a bacterium is an endospore ...
Page 513
... Endospore - forming rods and cocci , key to media and cultural requirements for , 87 Endospores , procedures for staining , 28-29 Enrichment and isolation of bacteria , 112-142 biochemical enrichment , 119-127 biological enrichment ...
... Endospore - forming rods and cocci , key to media and cultural requirements for , 87 Endospores , procedures for staining , 28-29 Enrichment and isolation of bacteria , 112-142 biochemical enrichment , 119-127 biological enrichment ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes