Manual of Methods for General Bacteriology |
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Page 24
... Ethanol , 95 % ( vol / vol ) , 20 ml Ammonium oxalate , 1 % ( wt / vol ) aqueous , 80 ml Dissolve the crystal violet in the ethanol . Then add the ammonium oxalate solution and allow to stand for 48 h before use . Methylene blue ...
... Ethanol , 95 % ( vol / vol ) , 20 ml Ammonium oxalate , 1 % ( wt / vol ) aqueous , 80 ml Dissolve the crystal violet in the ethanol . Then add the ammonium oxalate solution and allow to stand for 48 h before use . Methylene blue ...
Page 331
... ethanol to remove residual trichloroacetic acid , which may cause solubili- zation of some protein in the next step . Discard the filtrate . 3. Place the filter holder ( with the filter and suction flask ) and a test tube for collecting ...
... ethanol to remove residual trichloroacetic acid , which may cause solubili- zation of some protein in the next step . Discard the filtrate . 3. Place the filter holder ( with the filter and suction flask ) and a test tube for collecting ...
Page 332
... ethanol , to remove residual tri- chloroacetic acid that would otherwise solubilize some protein in the next step . 4. Wash the filter twice with 5 ml of 70 % ethanol ( 45 ° C ) and twice with 5.0 ml of ethanol- diethyl ether at 45 ° C ...
... ethanol , to remove residual tri- chloroacetic acid that would otherwise solubilize some protein in the next step . 4. Wash the filter twice with 5 ml of 70 % ethanol ( 45 ° C ) and twice with 5.0 ml of ethanol- diethyl ether at 45 ° C ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes