Manual of Methods for General Bacteriology |
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Page 140
0 g Yeast extract ( NH ) SO Tris ( hydroxymethyl ) aminomethane ( Tris buffer )
Distilled water 15 . 7 g 1 , 000 mi Prepare stock solutions of each ingredient sep -
arately and sterilize by autoclaving . ( The pH of the Tris buffer solution should be
...
0 g Yeast extract ( NH ) SO Tris ( hydroxymethyl ) aminomethane ( Tris buffer )
Distilled water 15 . 7 g 1 , 000 mi Prepare stock solutions of each ingredient sep -
arately and sterilize by autoclaving . ( The pH of the Tris buffer solution should be
...
Page 370
Chelating agents such as EDTA are An enzyme assay should , if possible , be
accuoften included in cell extracts to ... Among these general When an enzyme is
thought to be present in considerations are the following . a crude extract but the
...
Chelating agents such as EDTA are An enzyme assay should , if possible , be
accuoften included in cell extracts to ... Among these general When an enzyme is
thought to be present in considerations are the following . a crude extract but the
...
Page 435
3 g of KOH . Add the remaining ingredients , and adjust the pH to 6 . 8 . 20 . 3 . 19
. Leifson Modified 0 - F ( MOF ) Medium ( 55 ) Casitone ( Difco ) . . . . . 1 . 0 g
Yeast extract 0 . 1 g ( NH4 ) 2SO4 0 . 5 g Tris buffer ( 2 - amino - 2 -
hydroxymethyl1 ...
3 g of KOH . Add the remaining ingredients , and adjust the pH to 6 . 8 . 20 . 3 . 19
. Leifson Modified 0 - F ( MOF ) Medium ( 55 ) Casitone ( Difco ) . . . . . 1 . 0 g
Yeast extract 0 . 1 g ( NH4 ) 2SO4 0 . 5 g Tris buffer ( 2 - amino - 2 -
hydroxymethyl1 ...
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Contents
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
GROWTH RALPH N COSTILOW Editor | 63 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter Microbiol microscopy mixture mutants needed obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York