Manual of Methods for General Bacteriology |
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Page 27
... film with the iodine mordant and then cover the film with fresh iodine mor- dant for 2 min . 3. Wash the film with a gentle and indirect stream of tap water for 2 s ; blot around the film with absorbent paper , but do not allow the film ...
... film with the iodine mordant and then cover the film with fresh iodine mor- dant for 2 min . 3. Wash the film with a gentle and indirect stream of tap water for 2 s ; blot around the film with absorbent paper , but do not allow the film ...
Page 37
... films are obtained on the water surface , carefully place the grids ( dull side down ) onto the film . Avoid using film areas with obvious irregularities , in order to maintain a degree of consistency . 9. To retrieve the coated grids ...
... films are obtained on the water surface , carefully place the grids ( dull side down ) onto the film . Avoid using film areas with obvious irregularities , in order to maintain a degree of consistency . 9. To retrieve the coated grids ...
Page 45
... film cut to appropriate lengths , or separated stacks of cut film , are placed on a support grid for drying . A tray in the bottom contains phosphorus pentoxide , which has to be stirred at each opening in the darkroom and replaced as ...
... film cut to appropriate lengths , or separated stacks of cut film , are placed on a support grid for drying . A tray in the bottom contains phosphorus pentoxide , which has to be stirred at each opening in the darkroom and replaced as ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes