Manual of Methods for General Bacteriology |
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Page 155
... flask systems ( 10.1.2 . ) . Conditions that ensure strict anaerobic conditions for tube cultures are de- scribed elsewhere in this manual ( 6.6 ) . 10.1.2 . Shake Flasks An Erlenmeyer flask ( 100- to 2,000 - ml capac- ity ) commonly is ...
... flask systems ( 10.1.2 . ) . Conditions that ensure strict anaerobic conditions for tube cultures are de- scribed elsewhere in this manual ( 6.6 ) . 10.1.2 . Shake Flasks An Erlenmeyer flask ( 100- to 2,000 - ml capac- ity ) commonly is ...
Page 156
... flask with 50 ml of appropriate liquid medium . 2. Prepare the gauze pad as described above . 3. Fit the pad on the flask , secure it with a band or spring , and cover it with aluminum foil . 4. Press the foil around the cover and the ...
... flask with 50 ml of appropriate liquid medium . 2. Prepare the gauze pad as described above . 3. Fit the pad on the flask , secure it with a band or spring , and cover it with aluminum foil . 4. Press the foil around the cover and the ...
Page 433
... flask from side to side for 10 min . The fibrin formed during clotting will be deposited on the beads . Decant the ... flask equipped with a chimney ( to prevent boiling over ) . For a 1 - liter flask , use 750 ml of the broth ; for a ...
... flask from side to side for 10 min . The fibrin formed during clotting will be deposited on the beads . Decant the ... flask equipped with a chimney ( to prevent boiling over ) . For a 1 - liter flask , use 750 ml of the broth ; for a ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes