Manual of Methods for General Bacteriology |
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Page 60
... fraction ( Supernatant I , Fig . 1 ) at 21 ° C . BROKEN CELLS SUPERNATANT I Incubate at 21 ° C for 30 min Centrifuge at 200,000 × g for 1 h SUPERNATANT II ( CYTOPLASM AND PERI- PLASM ) Centrifuge at 200,000 × g for 1 h SEDIMENT I ...
... fraction ( Supernatant I , Fig . 1 ) at 21 ° C . BROKEN CELLS SUPERNATANT I Incubate at 21 ° C for 30 min Centrifuge at 200,000 × g for 1 h SUPERNATANT II ( CYTOPLASM AND PERI- PLASM ) Centrifuge at 200,000 × g for 1 h SEDIMENT I ...
Page 167
... fractions in which the most dense fraction contains both young ( daughter ) cells and mature ( ready to divide ) cells , while the least dense fraction will contain a homogeneous population of cells that have progressed through a common ...
... fractions in which the most dense fraction contains both young ( daughter ) cells and mature ( ready to divide ) cells , while the least dense fraction will contain a homogeneous population of cells that have progressed through a common ...
Page 329
... fraction ( 2 ) . The precipitate fraction of macromolecules is sequentially extracted with organic solvents for lipids , alkali for ribonucleic acids ( RNAs ) , and hot trichloroacetic acid for deoxyribonucleic acids ( DNAs ) ; the ...
... fraction ( 2 ) . The precipitate fraction of macromolecules is sequentially extracted with organic solvents for lipids , alkali for ribonucleic acids ( RNAs ) , and hot trichloroacetic acid for deoxyribonucleic acids ( DNAs ) ; the ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes