Manual of Methods for General Bacteriology |
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Page 329
The precipitate fraction of macromolecules is sequentially extracted with organic solvents for lipids , alkali for ribonucleic acids ( RNAs ) , and hot trichloroacetic acid for deoxyribonucleic acids ( DNAs ) ; the precipitate remaining ...
The precipitate fraction of macromolecules is sequentially extracted with organic solvents for lipids , alkali for ribonucleic acids ( RNAs ) , and hot trichloroacetic acid for deoxyribonucleic acids ( DNAs ) ; the precipitate remaining ...
Page 331
Determine the radioactivity of the hydroml of ice - cold 70 % ethanol to remove residual lyzed RNA fraction in the same way as for the trichloroacetic acid , which may cause solubili- small - molecule fraction ( see above and 16.4.3 ) ...
Determine the radioactivity of the hydroml of ice - cold 70 % ethanol to remove residual lyzed RNA fraction in the same way as for the trichloroacetic acid , which may cause solubili- small - molecule fraction ( see above and 16.4.3 ) ...
Page 332
This can be determined by fractionating the cells and estimating the radioactivity of each fraction by the techniques described above . If all of the radioactivity occurs in the fraction of interest , subsequent experiments can be ...
This can be determined by fractionating the cells and estimating the radioactivity of each fraction by the techniques described above . If all of the radioactivity occurs in the fraction of interest , subsequent experiments can be ...
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Contents
1 | 22 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino amount anaerobic applications appropriate assay autoclaving bacteria base broth buffer cells centrifuge chemical colonies column components concentration containing counting cover culture cytoplasmic described determine dilution Dissolve distilled water drop effective electron enzyme examination example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method microscopy mixture mutants needed objective obtained organisms oxygen plasmid plate positive possible prepared present Press procedure protein reaction reagent references remove sample selection separation slide sodium solution specific specimen staining standard sterile surface suspension Table techniques temperature tion transfer tube usually volume Wash York