Manual of Methods for General Bacteriology |
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Page 56
... fragments in the supernatant . Cell wall fragments and large membrane struc- tures can be pelleted by centrifugation at 20,000 to 50,000 × g for 20 min , whereas centrifugation at 200,000 × g for 1 h is required to pellet small membrane ...
... fragments in the supernatant . Cell wall fragments and large membrane struc- tures can be pelleted by centrifugation at 20,000 to 50,000 × g for 20 min , whereas centrifugation at 200,000 × g for 1 h is required to pellet small membrane ...
Page 60
... fragments to aggregate or fuse into larger membrane fragments which can then be sedimented ( 6 ) . In some cases , this non - sedi- mentable membrane fraction can represent as much as 20 to 30 % of the cytoplasmic membrane . Its ...
... fragments to aggregate or fuse into larger membrane fragments which can then be sedimented ( 6 ) . In some cases , this non - sedi- mentable membrane fraction can represent as much as 20 to 30 % of the cytoplasmic membrane . Its ...
Page 466
... fragments . When determining Cot1 / 2 val- ues , however , one must know these parameters . This is because a series of points between kCot = 0.1 and kCot 10 must be determined . In practice , for bacterial DNA preparations , these will ...
... fragments . When determining Cot1 / 2 val- ues , however , one must know these parameters . This is because a series of points between kCot = 0.1 and kCot 10 must be determined . In practice , for bacterial DNA preparations , these will ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes