Manual of Methods for General Bacteriology |
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Page 60
When cells are broken by shear forces , some of the cytoplasmic membrane
becomes frag . mented extensively ( probably into short , " open " fragments of
membrane ) and cannot be sedi - mented in the ultracentrifuge . This is the
reason for ...
When cells are broken by shear forces , some of the cytoplasmic membrane
becomes frag . mented extensively ( probably into short , " open " fragments of
membrane ) and cannot be sedi - mented in the ultracentrifuge . This is the
reason for ...
Page 464
Therefore , the extent of duplex formation Rad Laboratories , Richmond , Calif . ) .
Double - between radioactively labeled DNA fragments stranded DNA will
adsorb to hydroxylapatite in and an excess of unlabeled DNA fragments can 0 .
Therefore , the extent of duplex formation Rad Laboratories , Richmond , Calif . ) .
Double - between radioactively labeled DNA fragments stranded DNA will
adsorb to hydroxylapatite in and an excess of unlabeled DNA fragments can 0 .
Page 466
Fraction of DNA fragments that are single - stranded 0 . 1 10 . 0 Coty = 1 / X1 / 2 .
If the logs of the x values are plotted , when y = 12 , log a = log 1 / X1 / 2 = - log X1
/ 2 . A general log Cot plot is shown in Fig . 9 . There is an almost linear region ...
Fraction of DNA fragments that are single - stranded 0 . 1 10 . 0 Coty = 1 / X1 / 2 .
If the logs of the x values are plotted , when y = 12 , log a = log 1 / X1 / 2 = - log X1
/ 2 . A general log Cot plot is shown in Fig . 9 . There is an almost linear region ...
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Contents
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
GROWTH RALPH N COSTILOW Editor | 63 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter Microbiol microscopy mixture mutants needed obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York