Manual of Methods for General Bacteriology |
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Page 222
First , the occurrence of a mutation in a specific gene function allows both the
identi - fication of that gene and , together with genetic mapping analyses , the
localization of the gene on the chromosome . Second , the analysis of mutants ...
First , the occurrence of a mutation in a specific gene function allows both the
identi - fication of that gene and , together with genetic mapping analyses , the
localization of the gene on the chromosome . Second , the analysis of mutants ...
Page 243
Chapter 14 Gene Transfer ROY CURTISS III 14 . 1 . TRANSFORMATION . . . . . 14
. 1 . 1 . Isolation of DNA . . . . . . . . . . . . . . . . . . . . . . . . . . 2 . Quantitation of DNA 244 .
3 . Transformation in Acinetobacter calcoaceticus . . . . . . . 245 . 4 .
Chapter 14 Gene Transfer ROY CURTISS III 14 . 1 . TRANSFORMATION . . . . . 14
. 1 . 1 . Isolation of DNA . . . . . . . . . . . . . . . . . . . . . . . . . . 2 . Quantitation of DNA 244 .
3 . Transformation in Acinetobacter calcoaceticus . . . . . . . 245 . 4 .
Page 383
The binding of the hibited and cAMP production is curtailed , relac repressor ( i
gene product ) to the operator sulting in the ... site prevents ribonucleic acid
polymerase tran - catabolite repression . scription ( negative control ) of the lac
genes .
The binding of the hibited and cAMP production is curtailed , relac repressor ( i
gene product ) to the operator sulting in the ... site prevents ribonucleic acid
polymerase tran - catabolite repression . scription ( negative control ) of the lac
genes .
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Contents
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
GROWTH RALPH N COSTILOW Editor | 63 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter Microbiol microscopy mixture mutants needed obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York