Manual of Methods for General Bacteriology |
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Page 57
... gradient - forming de- vices are available , but these are unnecessary since satisfactory gradients can be prepared by layering a series of sucrose solutions in a centri- fuge tube and allowing the gradient to " age " overnight in order ...
... gradient - forming de- vices are available , but these are unnecessary since satisfactory gradients can be prepared by layering a series of sucrose solutions in a centri- fuge tube and allowing the gradient to " age " overnight in order ...
Page 230
... gradient plates ( 13.9.14 ) . 13.5.2 . REVERSION ANALYSIS ( FILTER DISK ) b . concentration by trial and error approaches can be time - consuming and difficult . In the first example given below , the use of a simple gra- dient plate ...
... gradient plates ( 13.9.14 ) . 13.5.2 . REVERSION ANALYSIS ( FILTER DISK ) b . concentration by trial and error approaches can be time - consuming and difficult . In the first example given below , the use of a simple gra- dient plate ...
Page 276
... gradient ( or in sucrose gradients ) by their banding charac- teristics . For example , dimers of two CCC DNA molecules will coband with CCC DNA in a CsCl- ethidium bromide gradient , but will sediment ahead of monomeric CCC DNA in a ...
... gradient ( or in sucrose gradients ) by their banding charac- teristics . For example , dimers of two CCC DNA molecules will coband with CCC DNA in a CsCl- ethidium bromide gradient , but will sediment ahead of monomeric CCC DNA in a ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes