Manual of Methods for General Bacteriology |
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Page 37
... grid while being retained over the grid bars and holes . Films that lift away from the filter paper with the grid , or do not break cleanly and evenly , are too thick ; some areas of the film may show this property while others are ...
... grid while being retained over the grid bars and holes . Films that lift away from the filter paper with the grid , or do not break cleanly and evenly , are too thick ; some areas of the film may show this property while others are ...
Page 38
... grid . ) 3. Allow the drop to remain for 1 min or longer to permit adherence of the bacteria or other particles to the film surface . Trial and error will determine the optimum time , which is also affected by the nature and charge ...
... grid . ) 3. Allow the drop to remain for 1 min or longer to permit adherence of the bacteria or other particles to the film surface . Trial and error will determine the optimum time , which is also affected by the nature and charge ...
Page 43
... grid ( section surface down ) on each rounded drop . Quickly cover the dish and allow to stain for 3 to 5 min . ( Experience will deter- mine the optimum time . ) 6. Remove each grid with tweezers and , while holding , immerse rapidly ...
... grid ( section surface down ) on each rounded drop . Quickly cover the dish and allow to stain for 3 to 5 min . ( Experience will deter- mine the optimum time . ) 6. Remove each grid with tweezers and , while holding , immerse rapidly ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes