Manual of Methods for General Bacteriology |
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Page 455
... hydroxylapatite procedure is that the RNA species are degraded to such an extent that they will not compete with DNA for adsorption sites on the hydroxyl- apatite . For some groups of organisms , RNase T1 may be required in addition to ...
... hydroxylapatite procedure is that the RNA species are degraded to such an extent that they will not compete with DNA for adsorption sites on the hydroxyl- apatite . For some groups of organisms , RNase T1 may be required in addition to ...
Page 464
... hydroxylapatite or the S1 nuclease procedure ( see below ) . Hydroxylapatite procedure . The hydroxylapatite procedure separates dou- ble - stranded DNA from single - stranded DNA by selective adsorption to hydroxylapatite ( Bio- Rad ...
... hydroxylapatite or the S1 nuclease procedure ( see below ) . Hydroxylapatite procedure . The hydroxylapatite procedure separates dou- ble - stranded DNA from single - stranded DNA by selective adsorption to hydroxylapatite ( Bio- Rad ...
Page 466
... hydroxylapatite or S1 nuclease pro- cedure ( 22.6.4 ) . Plot the fractions of DNA fragments not bound to hydroxylapatite or the fractions which are S1 nuclease sensitive on the y axis and Cot values on the x axis of four- to five ...
... hydroxylapatite or S1 nuclease pro- cedure ( 22.6.4 ) . Plot the fractions of DNA fragments not bound to hydroxylapatite or the fractions which are S1 nuclease sensitive on the y axis and Cot values on the x axis of four- to five ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes