Manual of Methods for General Bacteriology |
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Page 154
... lactose ( Fig . 2 ) . Rapid growth on glucose occurs first . At the point of glucose exhaustion , an inflection in the biomass curve occurs . ( There may even be a decline in biomass . ) A new en- zyme system for metabolism of lactose ...
... lactose ( Fig . 2 ) . Rapid growth on glucose occurs first . At the point of glucose exhaustion , an inflection in the biomass curve occurs . ( There may even be a decline in biomass . ) A new en- zyme system for metabolism of lactose ...
Page 232
... lactose - tetrazolium plates ( 13.9.3 ) so as to obtain 50 to 100 colonies per plate . 4. Incubate until colonies are 2 to 3 mm in diameter . The lactose - positive parents are de- tected as white colonies while the lactose - nega- tive ...
... lactose - tetrazolium plates ( 13.9.3 ) so as to obtain 50 to 100 colonies per plate . 4. Incubate until colonies are 2 to 3 mm in diameter . The lactose - positive parents are de- tected as white colonies while the lactose - nega- tive ...
Page 239
... Lactose - Tetrazolium Plates ( for de- tection of lactose - nonfermenting mutants ) Difco Antibiotic Medium no . 2 Distilled water 2,3,5 - Triphenyltetrazolium chloride ( Sigma Chemical Co. ) 950 ml 50 mg Dissolve the Antibiotic Medium ...
... Lactose - Tetrazolium Plates ( for de- tection of lactose - nonfermenting mutants ) Difco Antibiotic Medium no . 2 Distilled water 2,3,5 - Triphenyltetrazolium chloride ( Sigma Chemical Co. ) 950 ml 50 mg Dissolve the Antibiotic Medium ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes