Manual of Methods for General Bacteriology |
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Page 413
Cellulolytic Activity Method 1 ( 45 ) . This method of preparing cellulose gives the best form of native cellulose for testing cellulolytic activity ; for other methods , see references 12 and 13. Incorporate finely divided cellulose ...
Cellulolytic Activity Method 1 ( 45 ) . This method of preparing cellulose gives the best form of native cellulose for testing cellulolytic activity ; for other methods , see references 12 and 13. Incorporate finely divided cellulose ...
Page 416
Method 2. Streak a plate of blood agar base Positive test : liquefaction of at least a portion of ( without added blood ) . Pour an overlay of 10 ml the medium . Evaporation should be prevented of blood agar ( at 45 to 50 ° C ) onto the ...
Method 2. Streak a plate of blood agar base Positive test : liquefaction of at least a portion of ( without added blood ) . Pour an overlay of 10 ml the medium . Evaporation should be prevented of blood agar ( at 45 to 50 ° C ) onto the ...
Page 417
Method 2. This method is more sensitive than Method 1. Cut filter paper into strips 5 by 20 mm , and soak them in a solution of 5 % lead acetate . Sterilize the strips in cotton - stoppered test tubes and dry in a drying oven .
Method 2. This method is more sensitive than Method 1. Cut filter paper into strips 5 by 20 mm , and soak them in a solution of 5 % lead acetate . Sterilize the strips in cotton - stoppered test tubes and dry in a drying oven .
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Contents
1 | 22 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino amount anaerobic applications appropriate assay autoclaving bacteria base broth buffer cells centrifuge chemical colonies column components concentration containing counting cover culture cytoplasmic described determine dilution Dissolve distilled water drop effective electron enzyme examination example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method microscopy mixture mutants needed objective obtained organisms oxygen plasmid plate positive possible prepared present Press procedure protein reaction reagent references remove sample selection separation slide sodium solution specific specimen staining standard sterile surface suspension Table techniques temperature tion transfer tube usually volume Wash York