Manual of Methods for General Bacteriology |
From inside the book
Results 1-3 of 80
Page 277
... mixture and 0.2 ml of the reassociation mixture . The final re- agent concentrations are 0.1 mM ZnSO4 , 150 mM NaCl ( this includes the NaCl added with the 0.2 ml of the reassociation mixture ) , 30 mM sodium acetate buffer at pH 4.5 ...
... mixture and 0.2 ml of the reassociation mixture . The final re- agent concentrations are 0.1 mM ZnSO4 , 150 mM NaCl ( this includes the NaCl added with the 0.2 ml of the reassociation mixture ) , 30 mM sodium acetate buffer at pH 4.5 ...
Page 379
... mixture to 1.0 ml . To this , add 3.0 ml of 0.025 % 2,4 - dinitrophenylhydrazine in 0.5 M HCl , and allow the mixture to stand at room temperature for 15 min . Add 1 ml of 40 % KOH , shake the contents , and then read in a Klett ...
... mixture to 1.0 ml . To this , add 3.0 ml of 0.025 % 2,4 - dinitrophenylhydrazine in 0.5 M HCl , and allow the mixture to stand at room temperature for 15 min . Add 1 ml of 40 % KOH , shake the contents , and then read in a Klett ...
Page 390
... mixture of cold carrier compounds containing sufficient H2SO4 for a final pH of 1.5 to 1.8 . This stops further reactions and converts any metabolic acids present to their acid form . The cold carrier mixture ( for E. coli studies ) ...
... mixture of cold carrier compounds containing sufficient H2SO4 for a final pH of 1.5 to 1.8 . This stops further reactions and converts any metabolic acids present to their acid form . The cold carrier mixture ( for E. coli studies ) ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
13 other sections not shown
Other editions - View all
Common terms and phrases
acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes